Insulin is diluted with isotonic sodium chloride solution so that 0.1 ml of its solution contains 0.001 IU. To do this, 4 units are diluted in 40 ml of isotonic sodium chloride solution or water for injection; 1 ml of the resulting solution is diluted in 9 ml of isotonic sodium chloride solution or water.
Begin to inject with 0.1 ml intradermally into the forearm. Every 30 minutes, the introduction is repeated, doubling the concentration - 0.002, then 0.004 and 0.008 IU. On the 2nd day, 0.01, 0.02, 0.04 and 0.08 IU are administered, on the 3rd and 4th day - 0.25, 0.5, 1 and 2 IU. If allergic manifestations persist on the 2nd day, the dose is not increased, insulin is re-introduced at the same dosage.